Next-Generation Sequencing (NGS)

What is Next-Generation Sequencing (NGS)? Next-generation sequencing (NGS), also known as second-generation sequencing, refers to high-throughput DNA sequencing technologies that allow for the rapid and cost-effective analysis of DNA and RNA sequences. NGS is usually used for short molecules such as sequencing mRNA, copy number variants, and single nucleotide polymorphisms, and uses PCR.

 

Applications of Next-Generation Sequencing (NGS)

 

  • Non-invasive prenatal testing
  • HLA typing
  • Cancer screening
  • Diagnosis of infectious diseases
  • Diagnosis of genetic disorders
  • Forensic DNA analysis
  • Biomarker discovery
  • Pharmacogenomics
  • Drug target identification

Source: Hu, T., Chitnis, N., Monos, D. S., & Dinh, A. (2021). Next-generation sequencing technologies: An overview. Human Immunology, 82(11), 801–811. https://doi.org/10.1016/j.humimm.2021.02.012